|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15800||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameUbiquitin conjugating enzyme UbE2F
SpeciesH. sapiens (human)
Insert Size (bp)560
Entrez GeneUBE2F (a.k.a. NCE2)
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDEST17-UbE2F was a gift from Wade Harper (Addgene plasmid # 15800 ; http://n2t.net/addgene:15800 ; RRID:Addgene_15800)
For your References section:Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging. Jin J, Li X, Gygi SP, Harper JW. Nature. 2007 Jun 28. 447(7148):1135-8. 10.1038/nature05902 PubMed 17597759