pBindCY
(Plasmid
#158223)
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PurposeExpressing CheZ protein in response to blue light
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Depositing Lab
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Depositing OrganizationNational University of Singapore
Located in Singapore -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158223 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 158223-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 158223-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEK
- Backbone size w/o insert (bp) 2066
- Total vector size (bp) 2823
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCheZ
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SpeciesE. coli
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Insert Size (bp)642
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GenBank ID946392
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Tag
/ Fusion Protein
- YbaQ (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 158223-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 158223-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBindCY was a gift from Chueh Loo Poh (Addgene plasmid # 158223 ; http://n2t.net/addgene:158223 ; RRID:Addgene_158223) -
For your References section:
Blue Light-Directed Cell Migration, Aggregation, and Patterning. Zhang J, Luo Y, Poh CL. J Mol Biol. 2020 May 1;432(10):3137-3148. doi: 10.1016/j.jmb.2020.03.029. Epub 2020 Apr 2. 10.1016/j.jmb.2020.03.029 PubMed 32247761
Map uploaded by the depositor.
