human SLC12A5-V5-HA-2A-Luciferase repair template
(Plasmid #158576)

• Purpose: For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.
• Depositing Lab: Rudolf Jaenisch
• Publication: Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii: 11/503/eaau0164. doi: 10.1126/scitranslmed.aau0164.

Backbone

• Vector backbone: pEM021
• Backbone size w/o insert (bp): 4200
• Total vector size (bp): 8084
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm
• Species: H. sapiens (human)
• Insert Size (bp): 3881
• Entrez Gene: SLC12A5 (a.k.a. EIEE34, EIG14, KCC2, hKCC2)
• Tag / Fusion Protein:
  • V5 and HA on the endogenous KCC2 protein product

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: FseI (not destroyed)
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry