pPSG465
(Plasmid
#158587)
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PurposeEnzymes Bv-CYP76AD5, DODC, ARO4_K229L
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCustom
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameBv-CYP76AD5
Gene/Insert 2
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Gene/Insert nameDODC
Gene/Insert 3
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Gene/Insert nameARO4_K229L
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MutationK229L
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPSG465 was a gift from John Dueber (Addgene plasmid # 158587 ; http://n2t.net/addgene:158587 ; RRID:Addgene_158587) -
For your References section:
Peroxisome compartmentalization of a toxic enzyme improves alkaloid production. Grewal PS, Samson JA, Baker JJ, Choi B, Dueber JE. Nat Chem Biol. 2020 Oct 12. pii: 10.1038/s41589-020-00668-4. doi: 10.1038/s41589-020-00668-4. 10.1038/s41589-020-00668-4 PubMed 33046851