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pEF NS34a-msGFP-A'Q-mito
(Plasmid #158763)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 158763 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEF-GFP (#11154)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NS3/4a protease and msGFP
  • Species
    Hepatitis C virus
  • Mutation
    Flexible linkers between the protease and msGFP and msGFP and the cleavage site, ADVVCC/QMSY cleavage site

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

In-fusion cloning was used for constuction of this plasmid

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF NS34a-msGFP-A'Q-mito was a gift from Edwin Chapman (Addgene plasmid # 158763 ; http://n2t.net/addgene:158763 ; RRID:Addgene_158763)
  • For your References section:

    Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons. Vevea JD, Chapman ER. Elife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469. 10.7554/eLife.56469 PubMed 32515733