Skip to main content
Addgene

Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.

Vevea JD, Chapman ER
Elife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
158763pEF NS34a-msGFP-A'Q-mitoExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158764pEF NS34a-msGFP-'Q-mitoExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158765pEF NS34a-msGFP-'S-mitoExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158766pF(UG) hSyn NS34a-msGFP-A'Q-mitoLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158767pF(UG) hSyn NS34a-msGFP-'Q-mito Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158768pF(UG) hSyn NS34a-msGFP-'S-mitoLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158769pF(UG) CamKII sf iGluSnFRiGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.
158770pF(UG) hSyn SYP-SELFExpression of synaptophysin 1 variant with self-cleaving c-terminal tail.
158771pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

Antibodies from Article