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Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.
Vevea JD, Chapman ER
Elife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
158763pEF NS34a-msGFP-A'Q-mitoExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
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158764pEF NS34a-msGFP-'Q-mitoExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
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158765pEF NS34a-msGFP-'S-mitoExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
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158766pF(UG) hSyn NS34a-msGFP-A'Q-mitoLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
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158767pF(UG) hSyn NS34a-msGFP-'Q-mito Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
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158768pF(UG) hSyn NS34a-msGFP-'S-mitoLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
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158769pF(UG) CamKII sf iGluSnFRiGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.
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158770pF(UG) hSyn SYP-SELFExpression of synaptophysin 1 variant with self-cleaving c-terminal tail.
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158771pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)Expression of synaptotagmin 1 variant with self-cleaving C2 domain.
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Antibodies from Article