GSK3 alpha pMT2
(Plasmid #15896)

• Depositing Lab: Jim Woodgett
• Depositing Organization: Sinai Health System
  Located in Canada
• Publication: Woodgett et al EMBO J. 1990 Aug . 9(8):2431-8.
• Citations: 7 Articles

Backbone

• Vector backbone: pMT2
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GSK3 alpha
• Alt name: GSK3a
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Entrez Gene: GSK3A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pMT2-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • Research Use Only Terms (Nonprofits)
• Industry Terms:
  • Research Use Only Terms (Industry)

Depositor Comments

ORF is at 1189-2640.

DNA (Catalog # 15896-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • Research Use Only Terms (Nonprofits)
• Industry Terms:
  • Research Use Only Terms (Industry)

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 15896-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • Research Use Only Terms (Nonprofits)
• Industry Terms:
  • Research Use Only Terms (Industry)

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.