|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||159093||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerAndras Nagy
Vector typeMammalian Expression ; piggyBac
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid PB-CA retrofitted with an AgeI and HindIII linker to compare the piggyBac ITR's in that plasmid to piggyBac ITR's in pBluescript-PB (https://www.addgene.org/133885/).
The piggyBac ITR sequences in the plasmid PB-CA extends longer into the transposon sequence than the piggyBac ITR sequence in the plasmid pBluescript-PB (67 bp in 5' PB sequence and 44 bp in 3' PB sequence). The remaining PB ITR sequences in both constructs are otherwise identical.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PB-CA retrofitted was a gift from Mario Capecchi (Addgene plasmid # 159093 ; http://n2t.net/addgene:159093 ; RRID:Addgene_159093)