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Transgene Module (TrZ)
(Plasmid #15916)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15916 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 3800
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    SURE
  • Growth instructions
    Stratagene SURE Very Low copy plasmids--TLD recommends megaprep
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    800

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site BsmI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Clone your gene of interest into the Transgene Module and then move it into LTRi http://www.addgene.org/15913 or LTRi with NeoR http://www.addgene.org/16160

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Transgene Module (TrZ) was a gift from James Collins (Addgene plasmid # 15916)
  • For your References section:

    A tunable genetic switch based on RNAi and repressor proteins for regulating gene expression in mammalian cells. Deans TL, Cantor CR, Collins JJ. Cell. 2007 Jul 27. 130(2):363-72. 10.1016/j.cell.2007.05.045 PubMed 17662949