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Addgene

pBTSG
(Plasmid #159420)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159420 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBacMam
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    thermostable GFP (TGP)
  • Species
    Synthetic
  • Tag / Fusion Protein
    • polyhistidine (poly-His) (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full sequence is provided but the depositors have only verified the sequence of the multi-cloning site and the TGP-encoding region by DNA sequencing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTSG was a gift from Dianfan Li (Addgene plasmid # 159420 ; http://n2t.net/addgene:159420 ; RRID:Addgene_159420)
  • For your References section:

    An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification. Cai H, Yao H, Li T, Hutter CAJ, Li Y, Tang Y, Seeger MA, Li D. Commun Biol. 2020 Dec 10;3(1):753. doi: 10.1038/s42003-020-01478-z. 10.1038/s42003-020-01478-z PubMed 33303987