mCRISPRed pRSETB
(Plasmid #159454)

• Purpose: Expresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRed
• Depositing Lab: Oliver Griesbeck
• Publication: Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-621.e5. doi: 10.1016/j.chembiol.2020.02.004. Epub 2020 Mar 5.

Backbone

• Vector backbone: pRSETB
• Backbone manufacturer: Invitrogen/Thermofisher Scientific
• Backbone size w/o insert (bp): 2901
• Total vector size (bp): 3609
• Modifications to backbone: Increased His-Tag length to 10 x from the original 6 x
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCRISPRed
• Species: Entacmaea quadricolor
• Insert Size (bp): 708
• GenBank ID: MN844880.1 MN844880.1
• Promoter: T7
• Tag / Fusion Protein:
  • 10 x His-Tag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAATACGACTCACTATAGG
• 3′ sequencing primer: GCTTCCTTTCGGGCTTTGTTAGCAGCCGGATC

Resource Information

• Supplemental Documents:
  • mCRISPREd_pRSETB.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry