pLNCX1 HA Akt1
(Plasmid #15990)

• Depositing Lab: Morris Birnbaum
• Publication: Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

Backbone

• Vector backbone: pLNCX
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Akt1
• Alt name: PKB alpha
• Species: M. musculus (mouse)
• Insert Size (bp): 1200
• GenBank ID: NM_009652
• Entrez Gene: Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Alfonso Bellacosa, Fox Chase Cancer Center
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry