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pLNCX1 HA AKT2
(Plasmid #27295)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27295 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLNCX
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    protein kinase B beta
  • Alt name
    akt2
  • Alt name
    PKB beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Entrez Gene
    AKT2 (a.k.a. HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer AGC TCG TTT AGT GAA CCG TCA GAT CG
  • 3′ sequencing primer ACC TAC AGG TGG GGT CTT TCA TTC CC
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

There is a silent mutation at nt552 (CGG->CGA) of the depositor's seq. This mutation does not alter the amino acid sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLNCX1 HA AKT2 was a gift from Morris Birnbaum (Addgene plasmid # 27295 ; http://n2t.net/addgene:27295 ; RRID:Addgene_27295)