pLNCX1 HA AKT2
(Plasmid #27295)

• Depositing Lab: Morris Birnbaum
• Publication: Birnbaum lab plasmids (unpublished)

Backbone

• Vector backbone: pLNCX
• Backbone size w/o insert (bp): 6600
• Vector type: Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: protein kinase B beta
• Alt name: akt2
• Alt name: PKB beta
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Entrez Gene: AKT2 (a.k.a. HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: AGC TCG TTT AGT GAA CCG TCA GAT CG
• 3′ sequencing primer: ACC TAC AGG TGG GGT CTT TCA TTC CC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a silent mutation at nt552 (CGG->CGA) of the depositor's seq. This mutation does not alter the amino acid sequence.