Purposeoptogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||159967||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4543
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Insert Size (bp)1250
- Promoter hSyn1
- Cloning method Restriction Enzyme
- 5′ cloning site multiple; modular (not destroyed)
- 3′ cloning site multiple; modular (unknown if destroyed)
- 5′ sequencing primer 3'Syn-fwd
- 3′ sequencing primer WPRE- (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made by"optoJNKi" was from Addgene plasmid 89744.
Terms and Licenses
- Not Available to Industry
Constructs are multipart, modular and generated iteratively.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV-Synapsin-Ypet-optoJNKi-WPRE was a gift from Michael Courtney (Addgene plasmid # 159967 ; http://n2t.net/addgene:159967 ; RRID:Addgene_159967)
For your References section:Resonance energy transfer sensitises and monitors in situ switching of LOV2-based optogenetic actuators. Li LL, Klein FM, Li Greci L, Popinigis A, Freudenberg F, Courtney MJ. Nat Commun. 2020 Oct 9;11(1):5107. doi: 10.1038/s41467-020-18816-8. 10.1038/s41467-020-18816-8 PubMed 33037199