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Resonance energy transfer sensitises and monitors in situ switching of LOV2-based optogenetic actuators.
Li LL, Klein FM, Li Greci L, Popinigis A, Freudenberg F, Courtney MJ
Nat Commun. 2020 Oct 9;11(1):5107. doi: 10.1038/s41467-020-18816-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
159941AAV-synapsin-NLS-mTurquoise2-optoNES-WPREoptical regulation of nuclear export
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159942AAV-Synapsin-NLS-YPET-optoNES-WPREoptical regulation of nuclear export
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159943AAV-Synapsin-NLS-mScarlet-optoNES-WPREoptical regulation of nuclear export
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159944AAV-Synapsin-NLS-Dronpa-optoNES-WPREoptical regulation of nuclear export
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159945AAV-Synapsin-NLS-mTFP1.0-optoNES-WPREoptical regulation of nuclear export
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159946AAV-Synapsin-mTurquoise2-3XNLS-optoNES-WPREoptical regulation of nuclear export
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159947AAV-Synapsin-YPET-3XNLS-optoNES-WPREoptical regulation of nuclear export
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159948AAV-Synapsin-mScarlet-3XNLS-optoNES-WPREoptical regulation of nuclear export
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159949AAV-Synapsin-mTurquoise-3XNLS-WPREoptical regulation of nuclear export
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159950AAV-Synapsin-Ypet-3XNLS-WPREnucleus marker
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159951AAV-Synapsin-mCherry-3XNLS-WPREnucleus marker
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159952AAV-Synapsin-miRFP670-3XNLS-WPREnucleus marker
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159953AAV-Synapsin-mTurquoise2-C1-WPREuntargeted marker
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159954AAV-Synapsin-YPET-C1-WPREuntargeted marker
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159955AAV-Synapsin-mScarlet-C1-WPREuntargeted marker
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159956AAV-Synapsin-mCherry-C1-WPREuntargeted marker
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159957AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPREoptoNES construct with modified mTurquoise2-LOV2 RET
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159958AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPREoptoNES construct with modified mTurquoise2-LOV2 RET
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159959AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPREoptoNES construct with modified mTurquoise2-LOV2 RET
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159960AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPREoptoNES construct with modified mTurquoise2-LOV2 RET
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159961AAV-Synapsin-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPREoptoNES construct with modified mTurquoise2-LOV2 RET
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159962AAV-CMV-NLS-mTurquoise2-optoNES-WPREoptical regulation of nuclear export
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159963AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPREoptoNES construct with modified mTurquoise2-LOV2 RET
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159964AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPREoptoNES construct with modified mTurquoise2-LOV2 RET
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159965AAV-CMV-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPREoptoNES construct with modified mTurquoise2-LOV2 RET
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159966AAV-Synapsin-mTurquoise2-optoJNKi-WPREoptogenetic JNK inhibitor with increased RET to LOV2 to increase actuation sensitivity
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159967AAV-Synapsin-Ypet-optoJNKi-WPREoptogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation
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159968AAV-Synapsin-mScarlet-optoJNKi-WPREoptogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation
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159969AAV-Synapsin-mTurquoise2-optoJNKi-C450A-WPREnegative control (non-activatable) optogenetic JNK inhibitor
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159970AAV-Synapsin-mTurquoise2-optoJNKi-V416I-WPRE optogenetic JNK inhibitor with extended relaxation time
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159971AAV-Synapsin-mTurquoise2-optop38i3-WPREoptogenetic p38 inhibitor with RET to LOV2 to increase sensitivity
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159972AAV-Synapsin-mTurquoise2-optop38i5-WPREoptogenetic p38 inhibitor with RET to LOV2 to increase sensitivity
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159973AAV-Synapsin-NLS-YPET-optoNES.V416I-WPREoptical regulation of nuclear export with extended relaxation time
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159974AAV-Synapsin-NLS-YPET-optoNES.I427V-WPREoptical regulation of nuclear export with shorter relaxation time
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159975AAV-Synapsin-NLS-YPET-optoNES.F434L-WPREoptical regulation of nuclear export with shorter relaxation time
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159976AAV-Synapsin-JNKKTR -miRFP670-WPREsingle wavelength translocating reporter of the JNK pathway
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