Resonance energy transfer sensitises and monitors in situ switching of LOV2-based optogenetic actuators.
Li LL, Klein FM, Li Greci L, Popinigis A, Freudenberg F, Courtney MJ
Nat Commun. 2020 Oct 9;11(1):5107. doi: 10.1038/s41467-020-18816-8. PubMed Article
Li LL, Klein FM, Li Greci L, Popinigis A, Freudenberg F, Courtney MJ
Nat Commun. 2020 Oct 9;11(1):5107. doi: 10.1038/s41467-020-18816-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 159941 | AAV-synapsin-NLS-mTurquoise2-optoNES-WPRE | optical regulation of nuclear export |
| 159942 | AAV-Synapsin-NLS-YPET-optoNES-WPRE | optical regulation of nuclear export |
| 159943 | AAV-Synapsin-NLS-mScarlet-optoNES-WPRE | optical regulation of nuclear export |
| 159944 | AAV-Synapsin-NLS-Dronpa-optoNES-WPRE | optical regulation of nuclear export |
| 159945 | AAV-Synapsin-NLS-mTFP1.0-optoNES-WPRE | optical regulation of nuclear export |
| 159946 | AAV-Synapsin-mTurquoise2-3XNLS-optoNES-WPRE | optical regulation of nuclear export |
| 159947 | AAV-Synapsin-YPET-3XNLS-optoNES-WPRE | optical regulation of nuclear export |
| 159948 | AAV-Synapsin-mScarlet-3XNLS-optoNES-WPRE | optical regulation of nuclear export |
| 159949 | AAV-Synapsin-mTurquoise-3XNLS-WPRE | optical regulation of nuclear export |
| 159950 | AAV-Synapsin-Ypet-3XNLS-WPRE | nucleus marker |
| 159951 | AAV-Synapsin-mCherry-3XNLS-WPRE | nucleus marker |
| 159952 | AAV-Synapsin-miRFP670-3XNLS-WPRE | nucleus marker |
| 159953 | AAV-Synapsin-mTurquoise2-C1-WPRE | untargeted marker |
| 159954 | AAV-Synapsin-YPET-C1-WPRE | untargeted marker |
| 159955 | AAV-Synapsin-mScarlet-C1-WPRE | untargeted marker |
| 159956 | AAV-Synapsin-mCherry-C1-WPRE | untargeted marker |
| 159957 | AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE | optoNES construct with modified mTurquoise2-LOV2 RET |
| 159958 | AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPRE | optoNES construct with modified mTurquoise2-LOV2 RET |
| 159959 | AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE | optoNES construct with modified mTurquoise2-LOV2 RET |
| 159960 | AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE | optoNES construct with modified mTurquoise2-LOV2 RET |
| 159961 | AAV-Synapsin-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE | optoNES construct with modified mTurquoise2-LOV2 RET |
| 159962 | AAV-CMV-NLS-mTurquoise2-optoNES-WPRE | optical regulation of nuclear export |
| 159963 | AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE | optoNES construct with modified mTurquoise2-LOV2 RET |
| 159964 | AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE | optoNES construct with modified mTurquoise2-LOV2 RET |
| 159965 | AAV-CMV-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE | optoNES construct with modified mTurquoise2-LOV2 RET |
| 159966 | AAV-Synapsin-mTurquoise2-optoJNKi-WPRE | optogenetic JNK inhibitor with increased RET to LOV2 to increase actuation sensitivity |
| 159967 | AAV-Synapsin-Ypet-optoJNKi-WPRE | optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation |
| 159968 | AAV-Synapsin-mScarlet-optoJNKi-WPRE | optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation |
| 159969 | AAV-Synapsin-mTurquoise2-optoJNKi-C450A-WPRE | negative control (non-activatable) optogenetic JNK inhibitor |
| 159970 | AAV-Synapsin-mTurquoise2-optoJNKi-V416I-WPRE | optogenetic JNK inhibitor with extended relaxation time |
| 159971 | AAV-Synapsin-mTurquoise2-optop38i3-WPRE | optogenetic p38 inhibitor with RET to LOV2 to increase sensitivity |
| 159972 | AAV-Synapsin-mTurquoise2-optop38i5-WPRE | optogenetic p38 inhibitor with RET to LOV2 to increase sensitivity |
| 159973 | AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE | optical regulation of nuclear export with extended relaxation time |
| 159974 | AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE | optical regulation of nuclear export with shorter relaxation time |
| 159975 | AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE | optical regulation of nuclear export with shorter relaxation time |
| 159976 | AAV-Synapsin-JNKKTR -miRFP670-WPRE | single wavelength translocating reporter of the JNK pathway |