PurposePrime editing in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||159977||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesSynthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus
- Promoter CMV
/ Fusion Proteins
- BpSV40 NLS (N terminal on insert)
- BpSV40 NLS (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7-forward
- 3′ sequencing primer BGH-reverse (Common Sequencing Primers)
Please visit https://www.biorxiv.org/content/10.1101/2020.11.10.377580v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-PE2-NG was a gift from Yongsub Kim (Addgene plasmid # 159977 ; http://n2t.net/addgene:159977 ; RRID:Addgene_159977)
For your References section:Engineered Prime Editors with PAM flexibility. Kweon J, Yoon JK, Jang AH, Shin HR, See JE, Jang G, Kim JI, Kim Y. Mol Ther. 2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 10.1016/j.ymthe.2021.02.022. 10.1016/j.ymthe.2021.02.022 PubMed 33636398