pShuttle AMPK gamma1 H150R
(Plasmid
#15999)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15999 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepShuttle
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAMPK gamma1
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Alt namePrkag1
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SpeciesM. musculus (mouse)
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Insert Size (bp)1600
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MutationH150R
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GenBank IDNM_016781
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Entrez GenePrkag1 (a.k.a. AA571379, BB036179, Prkaac)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pShuttle AMPK gamma1 H150R was a gift from Morris Birnbaum (Addgene plasmid # 15999 ; http://n2t.net/addgene:15999 ; RRID:Addgene_15999)