pFLAG-CMV2-CARP1
(Plasmid
#16014)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16014 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFLAG-CMV2
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCARP1
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Alt nameRNF34
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Alt nameFYVE-RING finger protein
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SpeciesH. sapiens (human)
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Entrez GeneRNF34 (a.k.a. CARP-1, CARP1, RFI, RIF, RIFF, hRFI)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLAG-CMV2-CARP1 was a gift from Wafik El-Deiry (Addgene plasmid # 16014 ; http://n2t.net/addgene:16014 ; RRID:Addgene_16014) -
For your References section:
Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth. McDonald ER, El-Deiry WS. Proc Natl Acad Sci U S A. 2004 Apr 20. 101(16):6170-5. 10.1073/pnas.0307459101 PubMed 15069192