pCMV6 Myc PAK5 S573N
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16021||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2200
MutationS573N: constitutively active
Entrez GenePAK5 (a.k.a. PAK7)
/ Fusion Protein
- Myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI/BglII (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
Order of elements: SalI-Myc-XbaI-Pak5-EcoRI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV6 Myc PAK5 S573N was a gift from Jonathan Chernoff (Addgene plasmid # 16021 ; http://n2t.net/addgene:16021 ; RRID:Addgene_16021)
For your References section:p21-Activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phosphorylating BAD. Cotteret S, Jaffer ZM, Beeser A, Chernoff J. Mol Cell Biol. 2003 Aug . 23(16):5526-39. 10.1128/MCB.23.16.5526-5539.2003 PubMed 12897128