908H
(Plasmid #160240)

• Purpose: TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB
• Depositing Lab: Omar Akbari
• Publication: Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471. doi: 10.1111/imb.12717. Epub 2021 May 24.

Backbone

• Vector backbone: attB
• Backbone size w/o insert (bp): 7400
• Total vector size (bp): 14000
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Tet Operator (2x)
• Alt name: TetO
• Insert Size (bp): 55

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 2

• Gene/Insert name: hsp70 minimal promoter
• Alt name: hsp70 minimal promoter
• Species: D. melanogaster (fly)
• Insert Size (bp): 130

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 3

• Gene/Insert name: p-element promoter
• Alt name: p-element promoter
• Species: D. melanogaster (fly)
• Insert Size (bp): 249

Cloning Information for Gene/Insert 3

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 4

• Gene/Insert name: Firefly luciferase
• Alt name: Firefly luciferase
• Insert Size (bp): 2424
• Tag / Fusion Protein:
  • T2A GFP (C terminal on insert)

Cloning Information for Gene/Insert 4

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 5

• Gene/Insert name: Renilla luciferase
• Alt name: Renilla luciferase
• Insert Size (bp): 936

Cloning Information for Gene/Insert 5

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Gene/Insert 6

• Gene/Insert name: Ubiquitin promoter
• Alt name: Ubiquitin promoter
• Insert Size (bp): 1869

Cloning Information for Gene/Insert 6

• Cloning method: Gateway Cloning
• 5′ sequencing primer: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry