50 pLIB_STREP
(Plasmid
#160275)
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Purpose(Empty Backbone) N-term 3C cleavable 2xStrepII tag for insect cell expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLIB
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Modifications to backboneMCS altered for full compatibility within InteBac, N-terminal 3C-cleavable 2xStrepII tag added
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Vector typeInsect Expression
- Promoter polyhydrin
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Selectable markersGentamicin
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Tag
/ Fusion Protein
- 2xStrepII (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
50 pLIB_STREP was a gift from John Weir (Addgene plasmid # 160275 ; http://n2t.net/addgene:160275 ; RRID:Addgene_160275) -
For your References section:
InteBac - An integrated bacterial and baculovirus expression vector suite. Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR. Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957. 10.1002/pro.3957 PubMed 32955754
