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pGFAP::MyrGFP_v2
(Plasmid #160277)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 160277 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myrGFP
  • Insert Size (bp)
    700
  • Promoter Gfa2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Michael Brenner [mailto:[email protected]]

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a new version of Plasmid 22672: pGFAP::MyrGFP. A stop codon at the 3' end of myrEGFP prevents translation of the mouse Protamine 1 coding sequence downstream of the insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGFAP::MyrGFP_v2 was a gift from Robert Benezra (Addgene plasmid # 160277 ; http://n2t.net/addgene:160277 ; RRID:Addgene_160277)
  • For your References section:

    High levels of Id1 expression define B1 type adult neural stem cells. Nam HS, Benezra R. Cell Stem Cell. 2009 Nov 6. 5(5):515-26. 10.1016/j.stem.2009.08.017 PubMed 19896442