PurposeExpresses GFP from the Gfa2 promoter. This plasmid is a new version of Plasmid 22672 and includes a stop codon in the myrEGFP insert.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||160277||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)700
- Promoter Gfa2
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This is a new version of Plasmid 22672: pGFAP::MyrGFP. A stop codon at the 3' end of myrEGFP prevents translation of the mouse Protamine 1 coding sequence downstream of the insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGFAP::MyrGFP_v2 was a gift from Robert Benezra (Addgene plasmid # 160277 ; http://n2t.net/addgene:160277 ; RRID:Addgene_160277)
For your References section:High levels of Id1 expression define B1 type adult neural stem cells. Nam HS, Benezra R. Cell Stem Cell. 2009 Nov 6. 5(5):515-26. 10.1016/j.stem.2009.08.017 PubMed 19896442