pCAS-mak10 gRNA-MAK10Δ
(Plasmid #160384)

• Purpose: For CRISPR-mediated deletin og MAK10 in yeast
• Depositing Lab: John McCusker
• Publication: Yeast viruses (unpublished)

Backbone

• Vector backbone: pCAS-mak10 gRNA
• Backbone size w/o insert (bp): 8743
• Total vector size (bp): 9714
• Vector type: Yeast Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MAK10
• Alt name: YEL053C
• Species: S. cerevisiae (budding yeast)
• Promoter: PGK1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MAK10 was deleted in an S288C background with Cre-loxP. Lox-ed mak10 "deletion " fragment" consisting of sequences upstream and downstream of deleted ORF (~800bp) was amplified, adding XhoI restriction sites, and cloned into the vector. This insert serves as a donor template for making a MAK10 deletion. The vector is an all-in-one gRNA/Cas9/donor template vector for making a marker-free MAK10 deletion in yeast strains