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John McCusker Lab: Yeast viruses
Unpublished

Plasmids from Article

ID Plasmid Purpose  
160379pAG25-1199 cDNAN1199 narnavirus cDNA
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160380pGAL-flp(H305L)-NAToverexpression of Flp recombinase step-arrest mutant in yeast
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160381pESC-NAT-Ago-DcrOverexpression of Ago-Dcr fusion protein in yeast
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160382pGAL-SKI1-NATOverxpression of SKI1/XRN1 in yeast
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160384pCAS-mak10 gRNA-MAK10ΔFor CRISPR-mediated deletin og MAK10 in yeast
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160385pCAS-mak10 gRNAExpresses guide RNA for targeting MAK10 in yeast
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160386N1199 launchingExpression of N1199 narnavirus ORF under PGK1 promoter with a 3' HDV ribozyme sequence
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160388pGAL-PDE1Overxpression of PDE1 in yeast
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160389GFP-ATG8(416)/GFP-AUT7(416)Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection
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160390PJD1223Encodes L-A cDNA for curing L-A virus and Parent for pJD1223-NAT
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Antibodies from Article