pGAL-PDE1
(Plasmid
#160388)
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PurposeOverxpression of PDE1 in yeast
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160388 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLND46
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Backbone manufacturerGenetics. 2009 Sep; 183(1): 365–383.
- Backbone size w/o insert (bp) 3825
- Total vector size (bp) 6759
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Vector typeYeast Expression
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Selectable markersNourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePDE1
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Alt nameYGL248W
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3110
- Promoter GAL
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (destroyed during cloning)
- 3′ cloning site SpeI (destroyed during cloning)
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note plasmid contains the following mutations T114A, K153E, I201T and N306S in PDE1. Depositor confirms plasmid is functional.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGAL-PDE1 was a gift from John McCusker (Addgene plasmid # 160388 ; http://n2t.net/addgene:160388 ; RRID:Addgene_160388)
Map uploaded by the depositor.
