pVD1 M3_3-pTRNA-scf 2.1 (GB2075)
(Plasmid #160560)

• Purpose: tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
• Depositing Lab: Diego Orzaez
• Publication: Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 10.1111/pbi.13138. Epub 2019 May 23.

Backbone

• Vector backbone: pVD1
• Backbone manufacturer: self-made; derived from pGEMT-Easy manufactured by Promega
• Vector type: CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: M3_3-pTRNA-scf 2.1
• Species: Unspecified
• Insert Size (bp): 282
• Mutation: BsaI and BsmBI sites removed

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Supplemental Documents:
  • pVD1 M3_3-pTRNA-scf 2.1 (GB2075)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compatible with GoldenBraid; insert can be released with BsmBI