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Strong gene activation in plants with genome-wide specificity using a new orthogonal CRISPR/Cas9-based programmable transcriptional activator.
Selma S, Bernabe-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M, Garcia-Carpintero V, Granell A, Orzaez D
Plant Biotechnol J. 2019 Sep;17(9):1703-1705. doi: 10.1111/pbi.13138. Epub 2019 May 23.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
160558pVD1 M1_3-pTRNA-scf 2.1 (GB2073)tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
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160559pVD1 M2_3-pTRNA-scf 2.1 (GB2074)tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
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160560pVD1 M3_3-pTRNA-scf 2.1 (GB2075)tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
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160568pVD1 M1_1-pTRNA-scf 2.1 (GB2603)tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
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160570pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold
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160571pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.
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160593pUPD2 sgRNA sCRNA 2.0 (GB2461)Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.
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160609pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64
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160621pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold
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160622pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL
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160623pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains
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160624pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)
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160625pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold
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160626pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains
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160627pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains
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160645pDGB3 omega1 P35S:MS2:VPR:Tnos - P35S:dCas9:EDLL:Tnos (GB2085)Module for the expression of Ms2 protein fused to VPR and dCas9 fused to EDLL
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