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Addgene

pLX-B2 (GB3374)
(Plasmid #160636)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160636 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLX-B2
  • Backbone manufacturer
    Pasin et al., ACS Synth Biol. 2017;6(10):1962-1968. https://doi.org/10.1021/acssynbio.6b00354
  • Backbone size (bp) 3287
  • Vector type
    Synthetic Biology
  • Selectable markers
    lacZ

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GoldenBraid compatibility: enzyme in BsaI, enzyme out BsmBI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX-B2 (GB3374) was a gift from Juan Antonio García & Fabio Pasin (Addgene plasmid # 160636 ; http://n2t.net/addgene:160636 ; RRID:Addgene_160636)
  • For your References section:

    Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins. Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA. ACS Synth Biol. 2017 Oct 20;6(10):1962-1968. doi: 10.1021/acssynbio.6b00354. Epub 2017 Jun 28. 10.1021/acssynbio.6b00354 PubMed 28657330