pLX-B2 (GB3374)
(Plasmid
#160636)
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Purpose(Empty Backbone) pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160636 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLX-B2
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Backbone manufacturerPasin et al., ACS Synth Biol. 2017;6(10):1962-1968. https://doi.org/10.1021/acssynbio.6b00354
- Backbone size (bp) 3287
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Vector typeSynthetic Biology
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Selectable markerslacZ
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GoldenBraid compatibility: enzyme in BsaI, enzyme out BsmBI.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX-B2 (GB3374) was a gift from Juan Antonio García & Fabio Pasin (Addgene plasmid # 160636 ; http://n2t.net/addgene:160636 ; RRID:Addgene_160636) -
For your References section:
Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins. Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA. ACS Synth Biol. 2017 Oct 20;6(10):1962-1968. doi: 10.1021/acssynbio.6b00354. Epub 2017 Jun 28. 10.1021/acssynbio.6b00354 PubMed 28657330
