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pFRET-HSP33 cys
(Plasmid #16076)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16076 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 16076-D50 50 μg of DNA in Tris buffer $495
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DNA 16076-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pECFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5190
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HSP33 cys
  • Species
    E. coli
  • Insert Size (bp)
    900
  • Tags / Fusion Proteins
    • EYFP (N terminal on insert)
    • ECFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Ursula Jakob, Univ of MI

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The HSP-FRET probe was generated by inserting YFP into the pECFP-N1 plasmid between the NheI and BglII sites and then ligating the redox-sensitive regulatory domain from the Escherichia coli HSP-33 between YFP and CFP via the EcoRI and BamHI sites. HSP-33: 210 amino acids; BA000007 region 4241962-4242826. Made in Hammerling lab; used in Waypa et al., Increases in mitochondrial reactive oxygen species trigger hypoxia-induced calcium responses in pulmonary artery smooth muscle cells, Circ Res. 2006 Oct 27; 99(9):970-8 PMID: 17008601.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRET-HSP33 cys was a gift from Ulrich Hammerling (Addgene plasmid # 16076 ; http://n2t.net/addgene:16076 ; RRID:Addgene_16076)

  • For your References section:

    Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing. Guzy RD, Hoyos B, Robin E, Chen H, Liu L, Mansfield KD, Simon MC, Hammerling U, Schumacker PT. Cell Metab. 2005 Jun;1(6):401-8. doi: 10.1016/j.cmet.2005.05.001. 10.1016/j.cmet.2005.05.001 PubMed 16054089
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