PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||160881||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert namerbcS-S1, rbcX, raf1, raf2, bsd2
SpeciesA. thaliana (mustard weed); Nicotiana tabacum
- Promoter rbcS-S1: T7, chaperones: T7
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDF-NtXNtS1R1AtR2NtB2 was a gift from Maureen Hanson (Addgene plasmid # 160881 ; http://n2t.net/addgene:160881 ; RRID:Addgene_160881)
For your References section:Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli. Lin MT, Stone WD, Chaudhar Vi, Hanson MR. Nat Plants 10.1038/s41477-020-00761-5