PurposeExpression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||160876||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namerbcL, cpn60α, cpn60β, cpn20
SpeciesA. thaliana (mustard weed); Nicotiana tabacum
- Promoter rbcL: T7, chaperones: T7
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-AtC60αβC20NtL was a gift from Maureen Hanson (Addgene plasmid # 160876 ; http://n2t.net/addgene:160876 ; RRID:Addgene_160876)
For your References section:Small subunits can determine enzyme kinetics of tobacco Rubisco expressed in Escherichia coli. Lin MT, Stone WD, Chaudhar Vi, Hanson MR. Nat Plants 10.1038/s41477-020-00761-5