pCDF-NtXNtS1R1AtR2B2
(Plasmid #160880)

• Purpose: Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.
• Depositing Lab: Maureen Hanson
• Publication: Lin et al Nat Plants

Backbone

• Vector backbone: pCDF-1b
  (Search Vector Database)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rbcS-S1, rbcX, raf1, raf2, bsd2
• Species: A. thaliana (mustard weed); Nicotiana tabacum
• Promoter: rbcS-S1: T7, chaperones: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pCDF-NtXNtS1R1AtR2B2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry