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pMal-Abl-PRM 5R
(Plasmid #112088)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112088 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMal-tev
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 7035
  • Modifications to backbone
    tev site added on either side of NdeI / BamHI cloning sites, His-6 added to C-term after tev site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PRM-5R
  • Alt name
    5 repeats of the protein rich motif from proto-oncogene tyrosine-protein kinase (ABL)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    435
  • Mutation
    construct contains only the PRM motif of Abl1
  • GenBank ID
    AAB60394
  • Entrez Gene
    ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
  • Promoter Lac
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • His-6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pMAL-Fwd: 5'-TGCGTACTGCGGTGATCAAC-3'
  • 3′ sequencing primer pMAL-Rev: 5'-GTAAAACGACGGCCAGT-3'
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMal-Abl-PRM 5R was a gift from Michael Rosen (Addgene plasmid # 112088 ; http://n2t.net/addgene:112088 ; RRID:Addgene_112088)
  • For your References section:

    Phase transitions in the assembly of multivalent signalling proteins. Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, Rosen MK. Nature. 2012 Mar 7;483(7389):336-40. doi: 10.1038/nature10879. 10.1038/nature10879 PubMed 22398450