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Phase transitions in the assembly of multivalent signalling proteins.
Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, Rosen MK
Nature. 2012 Mar 7;483(7389):336-40. doi: 10.1038/nature10879.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
112086pMal_Abl-PRM 3RBacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.
112087pMAL-Abl-PRM 4RBacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.
112088pMal-Abl-PRM 5RBacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.
112089pMAL-SH3(2)-5RBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.
112090pGEX SH3(2)-4RBacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.
112091pGEX SH3(2)-3RBacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.
112154mCherry-SH3(5R)Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.
112155eGFP-PRM-5RMammalian expression plasmid for PRM-5R in a vector expressing eGFP.
112156eGFP-PRM-3RMammalian eGFP expression plasmid for PRM-3R.

Antibodies from Article