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pGEX SH3(2)-4R
(Plasmid #112090)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112090 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX2T
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6000
  • Modifications to backbone
    A Tev protease site has been added between the thrombin site and the multiple cloning site. The multiple cloning has been modified to begin with a NdeI (CATATG) site, and end with a BamHI (GGATCC) site.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SH3(2)-4R
  • Alt name
    NCK adaptor protein 1, isoform 2, second SH3 repeat x 4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    997
  • GenBank ID
    NP_001177725.1
  • Entrez Gene
    NCK1 (a.k.a. NCK, NCKalpha, nck-1)
  • Promoter Lac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3'
  • 3′ sequencing primer pGEX-3’: 5'-CCGGGAGCTGCATGTGTCAGAGG-3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are two NdeI sites - NdeI was used to make the additional repeats.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX SH3(2)-4R was a gift from Michael Rosen (Addgene plasmid # 112090 ; http://n2t.net/addgene:112090 ; RRID:Addgene_112090)
  • For your References section:

    Phase transitions in the assembly of multivalent signalling proteins. Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, Rosen MK. Nature. 2012 Mar 7;483(7389):336-40. doi: 10.1038/nature10879. 10.1038/nature10879 PubMed 22398450