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pWPXL-p150-PIP1Δ
(Plasmid #160993)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 160993 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CHAF1A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2721
  • Mutation
    PIP1 (PCNA-interacting protein) motif deletion
  • Entrez Gene
    Chaf1a (a.k.a. AL023013, AL024058, CAF-1, Cac1p)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWPXL-p150-PIP1Δ was a gift from Zhiguo Zhang (Addgene plasmid # 160993 ; http://n2t.net/addgene:160993 ; RRID:Addgene_160993)
  • For your References section:

    Chromatin Assembly Factor 1 (CAF-1) facilitates the establishment of facultative heterochromatin during pluripotency exit. Cheng L, Zhang X, Wang Y, Gan H, Xu X, Lv X, Hua X, Que J, Ordog T, Zhang Z. Nucleic Acids Res. 2019 Dec 2;47(21):11114-11131. doi: 10.1093/nar/gkz858. 10.1093/nar/gkz858 PubMed 31586391