|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||161515||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepDEST R4-R3
- Backbone size w/o insert (bp) 4107
- Total vector size (bp) 7663
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namepEXP[Psnt-1 | histamine | rpl-3 UTR]
SpeciesC. elegans (nematode)
- Promoter Psnt-1
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Synthetic Genome Biology lab @ KAUST www.wormbuilder.org
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSEM238 was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 161515 ; http://n2t.net/addgene:161515 ; RRID:Addgene_161515)
For your References section:A histamine-gated channel is an efficient negative selection marker for C. elegans transgenesis. El Mouridi, S; AlHarbi, S; Frøkjær-Jensen, C. microPublication Biology. 2021 10.17912/micropub.biology.000349