Skip to main content
Addgene
Open Menu

pM2AH + mSlo1
(Plasmid #16152)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16152 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 16152-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 16152-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pM2-AH
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mus musculus potassium large conductance calcium-activated channel
  • Alt name
    mSlo1
  • Alt name
    GeneID:16531
  • Alt name
    MGI:99923
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4740
  • Mutation
    N/A
  • GenBank ID
    NM_010610
  • Entrez Gene
    Kcnma1 (a.k.a. 5730414M22Rik, BKCA alpha, BKCa, KCa1.1, MaxiK, Slo, Slo1, k(VCA)alpha, mSlo, mSlo1, slo-alpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pM2AH + mSlo1 was a gift from Larry Salkoff (Addgene plasmid # 16152 ; http://n2t.net/addgene:16152 ; RRID:Addgene_16152)

  • For your References section:

    mSlo, a complex mouse gene encoding "maxi" calcium-activated potassium channels. Butler A, Tsunoda S, McCobb DP, Wei A, Salkoff L. Science. 1993 Jul 9. 261(5118):221-4. 10.1126/science.7687074 PubMed 7687074

Ready-to-use antibodies

Looking for antibodies related to this plasmid? Check out this option:

Learn More
Related items:
Commonly requested with: