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pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc
(Plasmid #161584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161584 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 161584-D50 50 μg of DNA in Tris buffer $495
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DNA 161584-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone size w/o insert (bp) 4902
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Depositor recommends growth at 30°C for 24 hrs.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lrrk2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    7700
  • Mutation
    D1994S
  • Entrez Gene
    Lrrk2 (a.k.a. 4921513O20Rik, 9330188B09Rik, D630001M17Rik, Gm927, cI-46)
  • Promoter CMV
  • Tags / Fusion Proteins
    • StrepII (N terminal on insert)
    • Myc tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site ApaI (unknown if destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG
  • 3′ sequencing primer GGC AAC TAG AAG GCA CAG TC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Neo cassette from pcDNA3.1(+) replaced with Puro cassette from pPUR (Clontech/TaKaRa)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc was a gift from Maik Hintze (Addgene plasmid # 161584 ; http://n2t.net/addgene:161584 ; RRID:Addgene_161584)
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