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(Plasmid #161587)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161587 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 4902
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Depositor recommends growth at 30°C for 24 hrs.
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    deletion: AA1023-1879
  • Entrez Gene
    Lrrk2 (a.k.a. 4921513O20Rik, 9330188B09Rik, D630001M17Rik, Gm927, cI-46)
  • Promoter CMV
  • Tags / Fusion Proteins
    • StrepII (N terminal on insert)
    • Myc tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site ApaI (unknown if destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG
  • 3′ sequencing primer GGC AAC TAG AAG GCA CAG TC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Neo cassette from pcDNA3.1(+) replaced with Puro cassette from pPUR (Clontech/TaKaRa)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc was a gift from Maik Hintze (Addgene plasmid # 161587 ; ; RRID:Addgene_161587)