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Addgene

pcDNA3.1(+)_Strep-Lrrk2-Myc
(Plasmid #161582)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161582 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5322
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Depositor recommends growth at 30°C for 24 hrs.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lrrk2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    7700
  • Mutation
    none
  • Entrez Gene
    Lrrk2 (a.k.a. 4921513O20Rik, 9330188B09Rik, D630001M17Rik, Gm927, cI-46)
  • Promoter CMV
  • Tags / Fusion Proteins
    • StrepII (N terminal on insert)
    • Myc tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site ApaI (unknown if destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG
  • 3′ sequencing primer GGC AAC TAG AAG GCA CAG TC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)_Strep-Lrrk2-Myc was a gift from Maik Hintze (Addgene plasmid # 161582 ; http://n2t.net/addgene:161582 ; RRID:Addgene_161582)

Ready-to-use antibodies

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