pUltra-Plus
(Plasmid #161775)

• Purpose: (Empty Backbone) 3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest
• Depositing Lab: Jorge Luis Arias-Arias
• Publication: pUltra series (unpublished)

Backbone

• Vector backbone: pUltra (Addgene Plasmid #24129)
• Backbone manufacturer: Malcolm Moore
• Backbone size (bp): 8326
• Modifications to backbone: Removal of EGFP coding sequence and SmaI restriction site / Insertion of EcoRV restriction site
• Vector type: Mammalian Expression, Lentiviral ; Multicistronic
• Promoter: UbC

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: hUBCpro-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pUltra-Plus was cloned by Jorge L. Arias-Arias at Universidad de Costa Rica ().

This vector allows the multicistronic expression of up to three genes in mammalian cells. The construct contains two 2A peptides coding sequences and three cloning positions flanked by restriction sites:

Position 1 (5' AgeI – 3' EcoRV)
Position 2 (5' XbaI – 3' BamHI)
Position 3 (5' NheI – 3' EcoRI or SaII)

The vector is suitable for transient transfection, but could also be used to produce lentiviral particles for stable expression in mammalian cells. For lentivirus packaging use the additional plasmids psPAX2 (Addgene Plasmid #12260) and pMD2.G (Addgene Plasmid #12259).