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pUltra-hot-H2B-mTagBFP2
(Plasmid #161792)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 161792 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUltra-hot (Addgene Plasmid #24130)
  • Backbone manufacturer
    Malcolm Moore
  • Backbone size w/o insert (bp) 8326
  • Total vector size (bp) 9412
  • Vector type
    Mammalian Expression, Lentiviral ; Multicistronic

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-mTagBFP2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1095
  • Mutation
    I174A in the mTagBFP2 protein according to PLoS ONE (2011) 6: e28674. doi:10.1371/journal.pone.0028674
  • GenBank ID
  • Promoter UbC
  • Tags / Fusion Proteins
    • mTagBFP2 (C terminal on insert)
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer hUBCpro-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pUltra-hot-H2B-mTagBFP2 was cloned by Jorge L. Arias-Arias at Universidad de Costa Rica ([email protected]).

This vector allows the multicistronic expression of mCherry, H2B-mTagBFP2, and one gene of interest in mammalian cells. The construct contains two 2A peptides coding sequences and two cloning positions flanked by restriction sites:

Position 1 (5' XbaI – 3' BamHI): Occupied by the H2B-mTagBFP2 insert.

Position 2 (5' NheI – 3' EcoRI or SaII): Available for cloning one gene of interest.

The vector is suitable for transient transfection, but could also be used to produce lentiviral particles for stable expression in mammalian cells. For lentivirus packaging use the additional plasmids psPAX2 (Addgene Plasmid #12260) and pMD2.G (Addgene Plasmid #12259).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUltra-hot-H2B-mTagBFP2 was a gift from Jorge Luis Arias-Arias (Addgene plasmid # 161792 ; http://n2t.net/addgene:161792 ; RRID:Addgene_161792)