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pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
(Plasmid #161928)


Item Catalog # Description Quantity Price (USD)
Plasmid 161928 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pAAVS1 donor
  • Modifications to backbone
    Replace mCherry Puro with mIFP. Replace U6 sgRNA with MCS.
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Surface marker and Citrine activation reporter

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned with reagents shared by and in collaboration with Michael Bassik.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA was a gift from Michael Bassik & Lacra Bintu (Addgene plasmid # 161928 ; ; RRID:Addgene_161928)
  • For your References section:

    High-Throughput Discovery and Characterization of Human Transcriptional Effectors. Tycko J, DelRosso N, Hess GT, Aradhana, Banerjee A, Mukund A, Van MV, Ego BK, Yao D, Spees K, Suzuki P, Marinov GK, Kundaje A, Bassik MC, Bintu L. Cell. 2020 Dec 9. pii: S0092-8674(20)31541-5. doi: 10.1016/j.cell.2020.11.024. 10.1016/j.cell.2020.11.024 PubMed 33326746