pBScMXT + Slo1
(Plasmid #16194)

• Depositing Lab: Larry Salkoff
• Depositing Organization: Washington University in Saint Louis
  Located in United States of America
• Publication: McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H767-77.

Backbone

• Vector backbone: pBScMXT
• Backbone size w/o insert (bp): 3200
• Vector type: Xenopus Oocyte expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: calcium-activated potassium channel
• Alt name: AAA92290
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 3900
• Mutation: N/A
• GenBank ID: U23767
• Entrez Gene: KCNMA1 (a.k.a. BKTM, CADEDS, IEG16, KCa1.1, LIWAS, MaxiK, PNKD3, SAKCA, SLO, SLO-ALPHA, SLO1, bA205K10.1, hSlo, mSLO1)
• Entrez Gene: Kcnma1 (a.k.a. 5730414M22Rik, BKCA alpha, BKCa, KCa1.1, MaxiK, Slo, Slo1, k(VCA)alpha, mSlo, mSlo1, slo-alpha)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Cla1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results do not exactly match the above GenBank accession numbers U23767 (nucleotide) and AAA92290 (protein) due to the cloning strategy employed for this plasmid. The beginning of the insert sequence from the Hind3 site (AAGCTT) to the Mlu1 site (ACGCGT) is from a mouse cDNA that contains an N-terminus that is highly conserved but not identical to that of the human channel. Prior to the starting Met of Slo1, this plasmid contains a short sequence from the mouse Slo1 5' untranslated region (5'UTR).

DNA (Catalog # 16194-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 16194-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.