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GFP-PIK3C2B
(Plasmid #161989)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 161989 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    eGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIK3C2B
  • Alt name
    accession number CAA74194
  • Species
    H. sapiens (human)
  • Mutation
    siRNA resistance to siRNA#2 with 4 silent mutations: 5’- GCTACCAGTTACGAGGACT-3’.
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid has also been described in: Wallroth, A., Koch, P.A., Marat, A.L., Krause, E., Haucke, V. (2019) Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signaling via mTORC1. Nature Cell Biol. 21, 1093-1101. doi: 10.1038/s41556-019-0377-3

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-PIK3C2B was a gift from David Bryant & Volker Haucke (Addgene plasmid # 161989 ; http://n2t.net/addgene:161989 ; RRID:Addgene_161989)
  • For your References section:

    mTORC1 activity repression by late endosomal phosphatidylinositol 3,4-bisphosphate. Marat AL, Wallroth A, Lo WT, Muller R, Norata GD, Falasca M, Schultz C, Haucke V. Science. 2017 Jun 2;356(6341):968-972. doi: 10.1126/science.aaf8310. 10.1126/science.aaf8310 PubMed 28572395