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PurposeBlue-light dependent heterodimerization system
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneM18 pCAGGS WPRE electroporation vector (Gray et al., 2016)
- Backbone size w/o insert (bp) 5897
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCytP450 ER targeting sequencel-EGFP-eMagA
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SpeciesSynthetic
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Insert Size (bp)1377
- Promoter beta-actin promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site HpaI (unknown if destroyed)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2020.08.28.272807 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ER-EGFP-eMagA was a gift from Pietro De Camilli (Addgene plasmid # 162245 ; http://n2t.net/addgene:162245 ; RRID:Addgene_162245) -
For your References section:
Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells. Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, De Camilli P. Elife. 2020 Nov 11;9. pii: 63230. doi: 10.7554/eLife.63230. 10.7554/eLife.63230 PubMed 33174843
