PurposeLevel 0 C-terminal tag part for MoClo assembly, TTCG-GCTT
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||162310||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeSynthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
Copy numberHigh Copy
Gene/Insert nameC-terminal tag, 9 aa linker, strep tag, stop codon
- Cloning method Unknown
- 5′ sequencing primer ggcgtatcacgaggcagaatttc
- 3′ sequencing primer CCTGCATAACGCGAAGTAATC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEPQD0CM0028 was a gift from Nicola Patron (Addgene plasmid # 162310 ; http://n2t.net/addgene:162310 ; RRID:Addgene_162310)
For your References section:Biofoundry-assisted expression and characterization of plant proteins. Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron NJ. Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 10.1093/synbio/ysab029. eCollection 2021. 10.1093/synbio/ysab029 PubMed 34693026