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Biofoundry-assisted expression and characterization of plant proteins.
Dudley QM, Cai YM, Kallam K, Debreyne H, Carrasco Lopez JA, Patron NJ
Synth Biol (Oxf). 2021 Sep 11;6(1):ysab029. doi: 10.1093/synbio/ysab029. eCollection 2021. PubMed Article

Plasmids from Article

ID Plasmid Purpose
162281pEPQD0KN0024Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis
162282pEPQD0KN0244Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis
162283pEPQD0KN0025Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis
162284pEPQD0KN0245Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis
162285pEPQD0CB0026Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System
162286pEPQD0CB0246Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System
162287pEPQD0KN0282Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System
162288pEPQD0KN0283Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System
162289pEPQD0KN0284Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System
162290pEPQD0KN0285Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System
162291pEPYC0CM0258 Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162292pEPQD0CM0541Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162293pEPQD0CM0542Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162294pEPQD0CM0543Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162295pEPQD0CM0544Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162296pEPQD0CM0545Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162297pEPQD0CM0546Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162298pEPQD0CM0547Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162299pEPQD0CM0548Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162300pEPQD0CM0549Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162301pEPQD0CM0550Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162302pEPQD0CM0281Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162303pEPQD0CM0551Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162304pEPMY0SP0002Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG
162305pEPQD0CM0296Level 0 CDS part for MoClo assembly, AATG-TTCG
162306pEPQD0CM0539Level 0 CDS part for MoClo assembly, AATG-TTCG
162307pEPYC0CM0134Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT
162308pEPYC0CM0257Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT
162309pEPQD0CM0027Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT
162310pEPQD0CM0028Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT
162311pEPQD0CM0029Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT
162312pEPQD0CM0030Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT
162313pEPQDKN0248Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT
162314pEPQDKN0332Reporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiT
162315pEPQDKN0329TEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT
162316pEPQDKN0729TEV protease plasmid for T7-driven E. coli cell-free protein synthesis
162317pEPMY1CB0001Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

Antibodies from Article