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Addgene

pJYDC1
(Plasmid #162458)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162458 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    New backbone pJYD
  • Backbone manufacturer
    Dr. Jiri Zahradnik
  • Backbone size (bp) 5169
  • Vector type
    Shuttle vector bacteria/yeast
  • Selectable markers
    TRP1
  • Tags / Fusion Proteins
    • eUnaG2 fluorescent protein (N terminal on backbone)
    • HA tag (N terminal on backbone)
    • HDEL sequence (C terminal on backbone)
    • myc tag (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJYDC1 was a gift from Gideon Schreiber (Addgene plasmid # 162458 ; http://n2t.net/addgene:162458 ; RRID:Addgene_162458)
  • For your References section:

    An enhanced yeast display platform demonstrates the binding plasticity under various selection pressures. Zahradník J, Dey D, Marciano S, Schreiber G. bioRxiv Preprint 2020 10.1101/2020.12.16.423176